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ULK3 Antibody

CAT: 0800-R32269Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32269Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells
UniProt
Q6PHR2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids HTEVQNLMARAEYLKEQVKMRESRWEAD of human ULK3 were used as the immunogen for the ULK3 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the ULK3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ULK3 antibody is available for research use only.
Storage Conditions
After reconstitution, the ULK3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ULK3 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of 1) rat brain, 2) rat testis, 3) mouse brain, and human 4) placenta, 5) 22RV1, and 6) SMMC lysate with ULK3 antibody. Predicted/observed molecular weight ~53 kDa.
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