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Nucb2 Antibody / Nucleobindin 2

CAT: 0800-R32171Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32171Size:100 µg
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Description
Nucleobindin-2 is a calcium-binding EF-hand protein that in humans is encoded by the NUCB2 gene. By exon trapping and sequence analysis, the NUCB2 sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (mouse protein tested) ; request BSA-free format for coating
UniProt
P81117
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
Amino acids 25-106 of mouse Nucleobindin 2 were used as the immunogen for the Nucb2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Nucb2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Nucb2 antibody is available for research use only.
Storage Conditions
After reconstitution, the Nucb2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Nucb2 antibody should be determined by the researcher.
Image Legend
Western blot testing of mouse HEPA cell lysate with Nucb2 antibody. Predicted molecular weight ~50 kDa.

UniProtKB · P81117

Nucleobindin-2

NUCB2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P81117
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Nucb2
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesPhosphoprotein

Cellular component

CytoplasmEndoplasmic reticulumGolgi apparatusMembraneNucleusSecreted

Molecular function

DNA-bindingGuanine-nucleotide releasing factor

Technical term

Reference proteome

Domain

RepeatSignal

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