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RBX1 Antibody / ROC1

CAT: 0800-R32161Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32161Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RING-box protein 1, also known as ROC1, is a protein that in humans is encoded by the RBX1 gene. This gene is mapped to chromosome 22q13.2 based on an alignment of the RBX1 sequence with the genomic sequence. ROC1 is recruited by cullin-1 to form a quaternary SCF (HOS) -ROC1 holoenzyme (with SKP1 and the BTRCP homolog HOS) . SCF (HOS) -ROC1 binds IKK-beta-phosphorylated I-kappa-B-alpha and catalyzes its ubiquitination in the presence of ubiquitin, E1, and CDC34. Conclusively, ROC1 plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P62877
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids NHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH of human ROC1/RBX1 were used as the immunogen for the RBX1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF/ICC, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the RBX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RBX1 antibody is available for research use only.
Storage Conditions
After reconstitution, the RBX1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RBX1 antibody should be determined by the researcher.
Image Legend
Western blot testing of 1) rat testis, 2) rat brain, 3) mouse brain and 4) mouse spleen lysate with RBX1 antibody. Expected molecular weight: 12-15 kDa.

UniProtKB · P62877

E3 ubiquitin-protein ligase RBX1

RBX1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P62877
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RBX1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.2.27, 2.3.2.32
Processing
—
Secondary accessions
B2RDY1, Q8N6Z8, Q9D1S2, Q9WUK9, Q9Y254
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Cellular component

CytoplasmNucleus

Biological process

DNA damageDNA repairHost-virus interactionUbl conjugation pathway

Ligand

Metal-bindingZinc

Molecular function

Transferase

Domain

Zinc-finger

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