Tissue Factor Antibody / TF / CD142

CAT: 0800-R31962Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R31962Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tissue factor also called platelet tissue factor, factor III, or CD142. The F3 gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
P13726
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids 33-295 of human TF/Factor III were used as the immunogen for the Tissue Factor antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.005% sodium azide
Reconstitution
After reconstitution, the Tissue Factor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Tissue Factor antibody is available for research use only.
Storage Conditions
After reconstitution, the Tissue Factor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Tissue Factor antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with Tissue Factor antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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