Tissue Factor Antibody / CD142 / F3

• Description:

  Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation Factor V (Factor V, FV, proaccelerin, labile factor) is a 2,196 amino acid, single chain glycoprotein that is cleaved by Thrombin to yield an active, Ca2+-dependent dimer that is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates into a prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Tissue factor (TF), also designated coagulation Factor III) is a cell surface glycoprotein that enables cells to initiate blood coagulation cascades. It functions as a high-affinity receptor for coagulation Factor VII.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  P13726

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 50-250 from human F3 protein was used as the immunogen for the Tissue Factor antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CD142/9195

• Applications:

  IHC-P, WB

• Purity:

  Protein G affinity

• Format:

  Purified

• Limitations:

  This Tissue Factor antibody is available for research use only.

• Storage Conditions:

  Aliquot the Tissue Factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Tissue Factor antibody should be determined by the researcher.

• Location:

  Secreted, membrane

• Image Legend:

  IHC staining of FFPE human tonsil tissue with Tissue Factor antibody (clone CD142/9195) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.