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DGAT Antibody

CAT: 0800-R31838Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31838Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) is a microsomal enzyme that plays a central role in the metabolism of cellular diacylglycerol lipids and catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT had been considered necessary for adipose tissue formation and essential for survival. There are two isozymes of DGAT encoded by the genes DGAT1 and DGAT2. DGAT1 is a host factor for HCV infection that binds core protein, localizes it to DGAT1-generated lipid droplets, and recruits viral RNA replication complexes for viral assembly. DGAT2-generated lipid droplets formed normally in cells treated with the DGAT1 inhibitor, suggesting that DGAT1 inhibitors may be useful as antiviral therapeutics.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL
UniProt
O75907
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Amino acids RRILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSR of human DGAT1 were used as the immunogen for the DGAT antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the DGAT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DGAT antibody is available for research use only.
Storage Conditions
After reconstitution, the DGAT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the DGAT antibody should be determined by the researcher.
Image Legend
Western blot testing of 1) rat kidney and 2) human HeLa lysate with DGAT antibody. Predicted molecular weight: ~57 kDa.

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