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P-Selectin Antibody / CD62P

CAT: 0800-R31776Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31776Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD62P is also known as SELP or P-Selectin. This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. P-Selectin redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. This membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-5 µg/mL, Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA free format for coating
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 42-271) was used as the immunogen for this P-Selectin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P, WB, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the P-Selectin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This P-Selectin antibody is available for research use only.
Storage Conditions
After reconstitution, the P-Selectin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the P-Selectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cell membrane
Image Legend
IHC staining of FFPE human spleen tissue with P-Selectin antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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