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MCM7 Antibody

CAT: 0800-R31674Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31674Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Minichromosome Maintenance 7, also called CDC47, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 526-719) was used as the immunogen for this MCM7 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
The lyophilized MCM7 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.
Limitations
This MCM7 antibody is available for research use only.
Storage Conditions
The lyophilized MCM7 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Variations in secondary/substrate sensitivities and test protocols may require the MCM7 antibody to be titrated for optimal performance.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human colon cancer tissue with MCM7 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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