MCM7 Antibody

• Description:

  Minichromosome Maintenance 7, also called CDC47, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human partial recombinant protein (AA 526-719) was used as the immunogen for this MCM7 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, ICC, IF, FACS

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  The lyophilized MCM7 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

• Limitations:

  This MCM7 antibody is available for research use only.

• Storage Conditions:

  The lyophilized MCM7 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Variations in secondary/substrate sensitivities and test protocols may require the MCM7 antibody to be titrated for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Immunofluorescent staining of FFPE human colon cancer tissue with MCM7 antibody. HIER: steam section in pH6 citrate buffer for 20 min.