Integrin alpha 5 Antibody

• Description:

  Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

• Specifications:

  Western blot: 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human partial recombinant protein (AA 787-992) was used as the immunogen for this Integrin alpha 5 antibody. Human Integrin alpha 5 shares 86% amino acid sequence identity with mouse Integrin alpha 5.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  The lyophilized Integrin alpha 5 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

• Limitations:

  This Integrin alpha 5 antibody is available for research use only.

• Storage Conditions:

  The lyophilized Integrin alpha 5 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Titration of the Integrin alpha 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Integrin alpha 5 antibody and Lane 1: rat brain; 2: mouse brain; 3: human MM231; 4: (h) HeLa; 5: (h) Jurkat; 6: (h) 293T; 7: (m) Neuro-2a; 8: (r) PC12 lysate; Expected molecular weight: 115-160 kDa, observed here at ~220 kDa.