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P27KIP1 Antibody

CAT: 0800-R31499Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31499Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cyclin-dependent kinase inhibitor 1B, also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of the protein is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunocytochemistry (FFPE) : 2-4 µg/mL
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Human partial recombinant protein (AA 10-198) was used as the immunogen for this p27KIP1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the p27KIP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This p27KIP1 antibody is available for research use only.
Storage Conditions
After reconstitution, the p27KIP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the p27KIP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of p27KIP1 antibody and Lane 1: rat thymus; 2: rat brain lysate. Expected/observed molecular weight ~27 kDa.

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