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CD34 Antibody

CAT: 0800-R31483Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31483Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD34 is a monomeric cell surface antigen with a glycosylated molecular mass of approximately 110 KD. It is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene is located on human chromosome 1. It plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 151-385) was used as the immunogen for this CD34 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the CD34 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD34 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD34 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the CD34 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
IHC staining of FFPE human glioma with CD34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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