ATG5 Antibody

CAT: 0800-R31384Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R31384Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of the protein in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These knockdown hepatocytes also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q9H1Y0
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 82-97 (DRFDQFWAINRKLMEY-human) were used as the immunogen for this ATG5 antibody (100% mouse homology) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the ATG5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ATG5 antibody is available for research use only.
Storage Conditions
After reconstitution, the ATG5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) human PANC-1, 2) human HT1080, 3) human U-251, 4) rat C6 and 5) mouse testis tissue lysate with ATG5 antibody. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.

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