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PIAS1 Antibody

CAT: 0800-R31376Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31376Size:100 µg
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Description
E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) ] family. It contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PIAS1 can also function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
O75925
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 636-651 (DTASIFGIIPDIISLD-human) were used as the immunogen for this PIAS1 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the PIAS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PIAS1 antibody is available for research use only.
Storage Conditions
After reconstitution, the PIAS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the PIAS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of PIAS1 antibody and Lane 1: rat testis; 2: mouse testis; 3: human HeLa; 4: (h) Jurkat; 5: (h) MCF-7; 6: (h) SKOV lysate; Expected size: 72~100KD

UniProtKB · O75925

E3 SUMO-protein ligase PIAS1

PIAS1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75925
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PIAS1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.2.-
Processing
—
Secondary accessions
B2RB67, B3KSY9, C5J4B4, Q147X4, Q99751, Q9UN02
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmCytoskeletonNucleus

Molecular function

DNA-bindingTransferase

Biological process

Host-virus interactionTranscriptionTranscription regulationUbl conjugation pathway

Ligand

Metal-bindingZinc

Domain

RepeatZinc-finger

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