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FLI1 Antibody

CAT: 0800-R31300Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31300Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Friend leukemia integration 1, also known as Transcription factor ERGB, is a protein that in humans is encoded by the human FLI1 gene. The protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event (s) associated with terminal differentiation to proliferation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
Q01543
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human Friend leukemia integration 1 (QQEWINQPVRVNVKREYDHM) was used as the immunogen for this FLI1 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the FLI1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This FLI1 antibody is available for research use only.
Storage Conditions
After reconstitution, the FLI1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the FLI1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of FLI1 antibody and Lane 1: Jurkat; 2: Raji cell lysate. Predicted/observed molecular weight: ~51kDa.
Product Datasheet
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