TRAF4 Antibody

• Description:

  TNF Receptor-Associated Factor 4, also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF4 gene. It is a member of the TNF receptor associated factor (TRAF) family, a family of scaffold proteins. Tomasetto et al. (1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al. (2002) generated Traf4-deficient mice by gene disruption.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

• UniProt:

  Q9BUZ4

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An amino acid sequence from the C-terminus of human TNF Receptor-Associated Factor 4 (PKFISHQDIRKRNYVRDD) was used as the immunogen for this TRAF4 antibody (100% homologous in human, mouse and rat) .

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the TRAF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This TRAF4 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the TRAF4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the TRAF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of TRAF4 antbody; Lane 1: rat thymus; 2: human HeLa; 3: (h) Jurkat; 4: mouse HEPA cell lysate. Predicted molecular weight: ~54 kDa.