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NM23 Antibody

CAT: 0800-R30927Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30927Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NME1 (NME/NM23 nucleoside diphosphate kinase 1), also called non-Metastatic protein 23 (NM23), is an enzyme that in humans encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NME1/NM23 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23 translocated to the nucleus and SET was degraded, allowing NM23 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al. (2003) showed that the Drosophila NME1 homolog, AWD, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells
UniProt
P15531
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human NM23 (EELVDYTSCAQNWIYE) was used as the immunogen for this NM23 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the NM23 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NM23 antibody is available for research use only.
Storage Conditions
After reconstitution, the NM23 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the NM23 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of rat lysate samples: 1) heart, 2) brain, 3) liver, 4) skeletal muscle and human lysate samples: 5) PANC-1, 6) HeLa, 7) SK-OV-3 and 8) COLO320. Predicted molecular weight ~17 kDa.

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