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NME2 Antibody / NM23-H2

CAT: 0800-V7488SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7488SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP) . In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P22392
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this NME2 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CPTC-NME2-2
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the NME2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NME2 antibody is available for research use only.
Storage Conditions
Store the NME2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic, nuclear
Image Legend
IHC staining of FFPE human breast carcinoma with NME2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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