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HSP70 Antibody

CAT: 0800-R30911Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30911Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HSPA1 (heat shock 70kDa protein 1A) also known as HSPA1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. Shimizu et al. (1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of HSP72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
P08107
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Amino acids 577-596 (VISWLDANTLAEKDEFEHKR-human) were used as the immunogen for this HSP70 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the HSP70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HSP70 antibody is available for research use only.
Storage Conditions
After reconstitution, the HSP70 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the HSP70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat brain, 4) rat RH35, 5) mouse liver, 6) mouse kidney, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with HSP70 antibody. Expected molecular weight ~70 kDa.

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