RelB Antibody

• Description:

  RelB (v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. By RT-PCR and immunocytochemical analyses, Clark et al. (1999) showed that protein expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al. (2008) found that overexpression of RelB, but not RelA, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classic pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using RelB -/- mice, they showed that the protein itself was required for RankL-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both RelB -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al. (2008) concluded that the alternative NF-kappa-B pathway, via RelB, plays an essential and unique role in RankL signaling toward osteoclast development.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  Q01201

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 393-413 (LPFTYLPRDHDSYGVDKKRKR-human) were used as the immunogen for this RelB antibody (100% mouse homology) .

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the Rel B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This RelB antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Rel B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the RelB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of RelB antibody and Lane 1: rat testis; 2: (h) HeLa; 3: (m) NIH3T3; 4: (h) Raji; 5: (m) HEPA cell lysate. Predicted size: 62-70KD