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SSR3 Antibody

CAT: 0800-R30894Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30894Size:100 µg
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Description
Signal sequence receptor, gamma is also known as SSR3 and TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross (2012) mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37) . Hartmann et al. (1993) found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL
UniProt
Q9UNL2
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Amino acids 8-23 (KQQSEEDLLLQDFSRN) were used as the immunogen for this SSR3 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, IF
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the SSR3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SSR3 antibody is available for research use only.
Storage Conditions
After reconstitution, the SSR3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the SSR3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic (ER)
Image Legend
Immunofluorescent staining of FFPE human placental tissue with SSR3 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q9UNL2

Translocon-associated protein subunit gamma

SSRG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9UNL2
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SSR3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
TRAP-gamma
EC number
—
Processing
—
Secondary accessions
B2R7D0, B4E2P2, D3DNK5, Q549M4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Cellular component

Endoplasmic reticulumMembrane

Domain

TransmembraneTransmembrane helix

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