SNAP23 Antibody

• Description :

  Synaptosomal-Associated Protein, 23-KD is a protein that in humans is encoded by the SNAP23 gene. The gene has 8 exons, with the initiation codon located in exon 2 and is mapped on 15q15.1-q15.2. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. The protein is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, it is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. It is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

• UniProt :

  O00161

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  An amino acid sequence from the C-terminus of human SNAP23 (DTNRDRIDIANARAKKLIDS) was used as the immunogen for this SNAP23 antibody. This sequence is common to isoforms 1 and 2.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, IHC-F, ICC

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution :

  After reconstitution, the SNAP23 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This SNAP23 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the SNAP23 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the SNAP23 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Image Legend :

  Western blot testing of SNAP23 antibody and Lane 1: rat spleen; 2: (r) testis; 3: (r) ovary; 4: human HeLa; 5: (h) MCF-7; 6: (h) SKOV; Expected molecular weight: ~23/18kDa (isoforms 1/2) .