Rab5 Antibody

CAT: 0800-R30866Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R30866Size:100 µg
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Description
Ras-associated protein 5A, also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. Tuberin functions as a RAB5GAP in vivo to negatively regulate RAB5-GTP activity in endocytosis. Ectopically expressed ALS2 localized with RAB5 and early endosome antigen-1 onto early endosomal compartments and stimulated the enlargement of endosomes in cultured cortical neurons. The protein is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP) . RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. Activation of the protein was either continuous or repetitive for up to 10 minutes, but it ended before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
P20339
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 138-155 (ANKRAVDFQEAQSYADDN) were used as the immunogen for this Rab5 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Rab5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Rab5 antibody is available for research use only.
Storage Conditions
After reconstitution, the Rab5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Rab5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of Rab5 antibody and Lane 1: rat brain; 2: rat ovary; 3: human placenta; 4: HeLa; 5: 293T; 6: A375; 7: COLO320; 8: MM453 cell lysate. Predicted/observed molecular weight: ~25kDa.

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