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IL-4 Antibody

CAT: 0800-R30841Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30841Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interleukin-4, also knowns as a B-cell stimulatory factor 1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
P05112
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the middle region of human Interleukin-4 (AATVLRQFYSHHEKDTR) was used as the immunogen for this IL-4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the IL-4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IL-4 antibody is available for research use only.
Storage Conditions
After reconstitution, the IL-4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Secreted
Image Legend
Western blot testing of IL-4 antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: human CCRF-CEM cell lysate. Expected molecular weight: 14-20 kDa depending on glycosylation level.

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