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MCM2 Antibody

CAT: 0800-R30734Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30734Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Minichromosome maintenance, S. cerevisiae, homolog of, 2, also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in two crucial steps of the cell cycle, namely, onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 2 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P49736
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human MCM2 (NKFSHDLKRKMILQQF) was used as the immunogen for this MCM2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the MCM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MCM2 antibody is available for research use only.
Storage Conditions
After reconstitution, the MCM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MCM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with MCM2 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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