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Bub3 Antibody

CAT: 0800-R30729Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R30729Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Budding uninhibited by benzimidazoles 3 is a protein that in humans is encoded by the BUB3 gene. It is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC) ; though Bub3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, it delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. It also acts as a regulator in that it affects binding of Mad3 to Mad2.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
O43684
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the middle region of human Bub3 (DHQLKMHDLNTDQEN) was used as the immunogen for this Bub3 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Bub3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Bub3 antibody is available for research use only.
Storage Conditions
After reconstitution, the Bub3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Bub3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Bub3 antibody and Lane 1: HeLa; 2: A549; 3: Jurkat; 4: COLO320 cell lysate

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