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Ku70 Antibody

CAT: 0800-R30726Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30726Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
XRCC6 (X-Ray Repair, Complementing Defective, In Chinese Hamster, 6), also called Ku70, G22P1 or TLAA, is a protein that in humans, is encoded by the XRCC6 gene. The XRCC6 gene encodesthe subunit p70 of the p70/p80 autoantigen which consists of two proteins of molecular mass of approximately 70,000 and 80,000 daltons that dimerize to form a 10 S DNA-binding complex. XRCC6 and Mre11 are differentially expressed during meiosis. XRCC6 interacts with Baxa, a mediator of mitochondrial-dependent apoptosis. Disruption of both FANCC and XRCC6/Ku70 suppressed sensitivity to crosslinking agents, diminished chromosome breaks, and reversed defective homologous recombination. Ku70 binds directly to free DNA ends, committing them to NHEJ repair. In early meiotic prophase, however, when meiotic recombination is most probably initiated, Mre11 was abundant, whereas Ku70 was not detectable.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P12956
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the C-terminus of human Ku70 (YPPDYNPEGKVTKRKH) was used as the immunogen for this Ku70 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Ku70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Ku70 antibody is available for research use only.
Storage Conditions
After reconstitution, the Ku70 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Ku70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human A549 cells with Ku70 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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