MDM2 Antibody

• Description:

  MDM2 is an important negative regulator of the p53 tumor suppressor. The protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al. (1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  Q00987

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 343-358 (EKAKLENSTQAEEGFD) were used as the immunogen for this MDM2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the MDM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MDM2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MDM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of MDM2 antibody and Lane 1: rat testis; 2: rat brain; 3: rat heart; 4: human SKOV-3; 5: COLO320; 6: HeLa; 7: mouse HEPA; 8: monkey COS7 cell lysate. Predicted molecular weight: ~55 kDa but can be observed at up to ~90 kDa.