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PD1 Antibody

CAT: 0800-R30141Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30141Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PDCD1 (Programmed cell death 1), also called PD1, encodes a cell surface receptor that is a member of the B7 superfamily involved in immunomodulation. This gene is mapped to 2q37.3. PDCD1 acts as an inhibitory molecule on T cells after interacting with its ligands PDL1 and PDL2. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. Using flow cytometric analysis, it has been found that expression of PDCD1 was upregulated on CD16-positive and CD16-negative monocytes, but not on dendritic cells, in viremic HIV-positive patients, but not in highly active antiretroviral therapy (HAART) -treated HIV-positive patients. Upregulation in monocytes was induced by microbial Toll-like receptor ligands and inflammatory cytokines.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-5 µg/mL
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 101-288) was used as the immunogen for this PD1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PD1 antibody is available for research use only.
Storage Conditions
After reconstitution, the PD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the PD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC staining of FFPE human tonsil tissue with PD1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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