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Transferrin Antibody

CAT: 0800-R30124Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30124Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. The protein is composed of alpha helices and beta sheets to form two domains. The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h) . Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe (III) binding sites. The affinity of transferrin for Fe (III) is extremely high but decreases progressively with decreasing pH below neutrality.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL (human protein tested)
UniProt
P02787
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 20-698) was used as the immunogen for this Transferrin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Transferrin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Transferrin antibody is available for research use only.
Storage Conditions
After reconstitution, the Transferrin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Transferrin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC staining of FFPE mouse liver tissue with Transferrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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