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Vimentin Antibody

CAT: 0800-R30090Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30090Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. The Vimentin gene expresses in human lymphocytes and in Burkitts lymphoma cells and it is secreted by activated macrophages. The gene encoding human protein is located on the short arm of chromosome 10.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL
Host
Mouse
Reactivity
Human, Rabbit, Pig, Rat
Immunogen
Pig eye lens vimentin was used as the immunogen for this Vimentin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
V9
Applications
WB, IHC-P, IF
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Vimentin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Vimentin antibody is available for research use only.
Storage Conditions
After reconstitution, the Vimentin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, nuclear, cell membrane
Image Legend
Immunofluorescent staining of FFPE human intestinal cancer with Vimentin antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA for 20 min.

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