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Paxillin Antibody

CAT: 0800-R30070Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30070Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunocytochemistry: Suitable
Host
Mouse
Reactivity
Human, Mouse, Rat, Chicken
Immunogen
C-terminal partial recombinant chicken paxillin (amino acids 305-559) was used as the immunogen for this Paxillin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
PXC-10
Applications
WB, ICC
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Paxillin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Paxillin antibody is available for research use only.
Storage Conditions
After reconstitution, the Paxillin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Paxillin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Paxillin antibody and Lane 1: 293T; 2: HeLa; 3: MCF-7; 4: MM231; 5: Jurkat cell lysate. Predicted molecular weight: 61/65/66 kDa (alpha/beta/gamma) .

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