MAP1 Antibody

• Description:

  Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs) . MAP1 is one of the major neuronal MAPs as well as being the largest (350KD) . MAPs include MAP1A, MAP1B, and MAP2. MAP1a is a single-copy gene spanning 10.5 kb. MAP1a coding sequence is contained in five exons. MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain.

• Specifications:

  Western blot: 0.5-2 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL--formalin or acetone fixed tissues

• Host:

  Mouse

• Reactivity:

  Mouse, Rat

• Immunogen:

  Rat brain microtubule-associated proteins were used as the immunogen for this MAP1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  HM-1

• Applications:

  WB, IHC-P, IHC-F

• Purity:

  Ascites

• Format:

  Ascites

• Buffer:

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution:

  After reconstitution, the MAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MAP1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MAP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the MAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC-P: MAP1 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer, HRP-staining.