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CD4 Antibody

CAT: 0800-R30015Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30015Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD stands for cluster of differentiation; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T, gp55]. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
CAS Number
9007-83-4
Specifications
IHC (Frozen) : 1 µg/mL-formalin or acetone fixed tissues, FACS: 1-3ug/10^6 cells
Host
Mouse
Reactivity
Human
Immunogen
CD4-transfected mouse T-cell hybridoma (3DT) followed by CD4+ human T-cell CEM cells was used as the immunogen for this CD4 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
CA-4
Applications
IHC-F, FACS
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the CD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD4 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the CD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Flow cytometry testing of human PBMC with CD4 antibody at 1ug/10^6 cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue=CD4 antibody.

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