Products for Research Use Only

Recombinant mTOR Antibody

CAT: 0800-R20291-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20291-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant mTOR antibody reacts to human serine/threonine-protein kinase mTOR. It may also react to mouse or rat mTOR, as predicted by immunogen homology.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1:500-1:1000 (1), Western blot: 1:1000-1:2000
UniProt
P42345
Host
Rabbit
Reactivity
Human
Immunogen
A peptide corresponding to the human serine/threonine-protein kinase mTOR was used as the immunogen for this recombinant mTOR antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM274
Type
Recombinant
Applications
WB, IHC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant mTOR antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant mTOR antibody is available for research use only.
Storage Conditions
Store the recombinant mTOR antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant mTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Prediction Reactivity
Mouse, Rat
Location
Cytoplasmic
Image Legend
Western blot of human HeLa cell lysate using recombinant mTOR antibody at 1:1500. Predicted molecular weight ~280 kDa, also observed at ~220 kDa.