MTOR Antibody

• Description:

  The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin and FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P42345

• Host:

  Rabbit

• Reactivity:

  Human, Monkey

• Immunogen:

  Amino acids N2093-N2537 from the human protein were used as the immunogen for the mTOR antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Limitations:

  This mTOR antibody is available for research use only.

• Storage Conditions:

  Store the mTOR antibody at -20°C.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the mTOR antibody should be determined by the researcher.

• Image Legend:

  IHC staining of FFPE human colon carcinoma with mTOR antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.