Recombinant mTOR Antibody

• Description:

  This recombinant mTOR antibody reacts to human serine/threonine-protein kinase mTOR. It may also react to mouse or rat mTOR, as predicted by immunogen homology.

• Specifications:

  Immunohistochemistry (FFPE) : 1:500-1:1000 (1), Western blot: 1:1000-1:2000

• UniProt:

  P42345

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A peptide corresponding to the human serine/threonine-protein kinase mTOR was used as the immunogen for this recombinant mTOR antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  RM274

• Type:

  Recombinant

• Applications:

  WB, IHC

• Purity:

  Protein A purified from animal origin-free supernatant

• Format:

  Purified

• Buffer:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Reconstitution:

  Store the recombinant mTOR antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

• Limitations:

  This recombinant mTOR antibody is available for research use only.

• Storage Conditions:

  Store the recombinant mTOR antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

• Formulation:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the recombinant mTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

• Prediction Reactivity:

  Mouse, Rat

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic

• Image Legend:

  Western blot of human HeLa cell lysate using recombinant mTOR antibody at 1:1500. Predicted molecular weight ~280 kDa, also observed at ~220 kDa.