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Recombinant CK8 Antibody

CAT: 0800-R20283-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20283-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant CK8 antibody reacts to human CK8/Cytokeratin-8.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1:1000-1:2000 (1), Western blot: 1:1000-1:2000
UniProt
P05787
Host
Rabbit
Reactivity
Human
Immunogen
A peptide corresponding to the C-terminus of human Cytokeratin 8 was used as the immunogen for this recombinant CK8 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM266
Type
Recombinant
Applications
WB, IHC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant CK8 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant CK8 antibody is available for research use only.
Storage Conditions
Store the recombinant CK8 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant CK8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Image Legend
Western blot testing of human HeLa cell lysate with recombinant CK8 antibody at 1:1000. Predicted molecular weight ~56 kDa.

UniProtKB · P05787

Keratin, type II cytoskeletal 8

K2C8_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05787
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT8
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K4H3, B0AZN5, F8VXB4, Q14099, Q14716, Q14717, Q53GJ0, Q6DHW5, Q6GMY0, Q6P4C7, Q96J60
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondMethylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Domain

Coiled coil

Cellular component

CytoplasmCytoskeletonIntermediate filamentKeratinNucleus

Disease

Disease variant

Biological process

Host-virus interaction

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