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Recombinant E-Cadherin Antibody

CAT: 0800-R20265-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20265-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant E-Cadherin antibody reacts to human E-cadherin/Cadherin-/CDH1.
CAS Number
9000-83-3
Specifications
Immunohistochemistry: 1:500-1:1000 (1), Western blot: 1:1000-1:2000
UniProt
P12830
Host
Rabbit
Reactivity
Human
Immunogen
A peptide corresponding to E-Cadherin/CDH1 was used as the immunogen for this recombinant E-Cadherin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM244
Type
Recombinant
Applications
WB, IHC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant E-Cadherin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant E-Cadherin antibody is available for research use only.
Storage Conditions
Store the recombinant E-Cadherin antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Location
Membrane, cytoplasmic
Image Legend
Western blot of human MCF7 lysate using recombinant E-Cadherin antibody at 1:1000. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on glycosylation level) .

UniProtKB · P12830

Cadherin-1

CADH1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P12830
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CDH1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A8K1U7, Q13799, Q14216, Q15855, Q16194, Q4PJ14, Q9UII8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCytoplasmEndosomeGolgi apparatusMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinUbl conjugation

Disease

Disease variantEctodermal dysplasia

Domain

RepeatSignalTransmembraneTransmembrane helix

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