CYLD Antibody

• Description:

  CYLD is involved in multiple physiological processes including immune response, osteoclastogenesis and spermatogenesis. It is a tumor suppressor via its negative regulation of NF-kB activation. CYLD is a deubiquitinase, removing ubiquitin chains from tumor-necrosis factor receptor-associated factor 2 (TRAF2) and thereby preventing its activation of IkB, a regulator of NF-kB. NF-kB remains sequestered in the cytoplasm, unable to translocate to the nucleus and initiate cell survival and oncogenetic signaling.

• Specifications:

  Western blot: 2-5 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A recombinant protein fragment from the N-termiinal region of human CYLD was used as the immunogen for this antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Aliquot the CYLD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CYLD antibody is available for research use only.

• Storage Conditions:

  Aliquot the CYLD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Provided assay concentrations are suggestions only, CYLD antibody titration may be required for optimal results.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human samples with CYLD antibody at 2ug/ml. Predicted molecular weight ~107kDa.