S100A8 Antibody

• Description:

  The S100 family of proteins are found in vertebrates and are characterized by two calcium-binding sites that have a helix-loop-helix conformation. The family of 24 is divided into three subgroups based on function: those with intracellular, intracellular plus extracellular, and extracellular regulatory effects. The calcium-binding protein S100A8, also called MRP8 or Myeloid-related protein 8, and Calgranulin A, forms a heterodimer with S100A9, called Calprotectin. The heterodimer is expressed by granulocytes, monocytes, and early differentiation states of macrophages, and is known to be an important pro-inflammatory mediator in acute and chronic inflammation. S100A8 and 9 are DAMPs, or Damage-associated molecular pattern molecules, and are able to activate receptors of the innate immune system, one being TLR4.

• Specifications:

  Western blot: 2-4 µg/mL

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant protein fragment from the N-terminal region of human S100A8 was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SA6279

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Aliquot the S100A8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This S100A8 antibody is available for research use only.

• Storage Conditions:

  Aliquot the S100A8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Provided assay concentrations are suggestions only, S100A8 antibody titration may be required for optimal results.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human samples with S100A8 antibody (clone SA6279) at 2ug/ml. Predicted molecular weight ~11 kDa.