S100A4 Antibody

CAT: 0800-V4030-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V4030-100UGSize:100 µg
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Description
S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P26447
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 1-200 from the human S100A4 protein was used as the immunogen for the S100A4 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
S100A4/7096
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the S100A4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This S100A4 antibody is available for research use only.
Storage Conditions
Aliquot the S100A4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the S100A4 antibody should be determined by the researcher.
Location
Cytoplasm, nucleus
Image Legend
IHC staining of FFPE human spleen tissue with S100A4 antibody (clone S100A4/7096) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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