S100A4 Antibody

• Description:

  S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

• Specifications:

  Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P26447

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 1-200 from the human S100A4 protein was used as the immunogen for the S100A4 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  S100A4/7096

• Applications:

  WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the S100A4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This S100A4 antibody is available for research use only.

• Storage Conditions:

  Aliquot the S100A4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the S100A4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, nucleus

• Image Legend:

  IHC staining of FFPE human spleen tissue with S100A4 antibody (clone S100A4/7096) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.