Caspase-3 Antibody, Pro and Active

• Description:

  Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12) . Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.

• Specifications:

  Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 5 µg/mL

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CAS3836

• Applications:

  WB, IHC-P

• Purity:

  Protein G purified monoclonal antibody

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Aliquot the Pro and Active Caspase-3 antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

• Limitations:

  This Caspase-3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Pro and Active Caspase-3 antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.

• Formulation:

  0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Western blot testing of staurosporine-treated Jurkat cells (2 hr, 2 uM) with Caspase-3 antibody at 2ug/ml. The pro form is seen at ~32kD and active caspase-3 seen at ~17 kDa and ~12 kDa.