MAPK3 Antibody / ERK1

• Description:

  ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2) . ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.

• Specifications:

  Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

• UniProt:

  P27361

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A portion of amino acids 1-30 from the human protein was used as the immunogen for the MAPK3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IHC-P, WB

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the MAPK3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MAPK3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the MAPK3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the MAPK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  Flow cytometry testing of human HeLa cells with MAPK3 antibody; Blue=isotype control, Green= MAPK3 antibody.