Prohibitin 2 Antibody / PHB2

• Description:

  PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER) -selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.

• Specifications:

  Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

• UniProt:

  Q99623

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A portion of amino acids 225-255 from the human protein was used as the immunogen for the Prohibitin 2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC-P, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Prohibitin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Prohibitin 2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Prohibitin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Prohibitin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  IHC testing of FFPE human colon tissue with Prohibitin 2 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.