G6PC Antibody

• Description:

  Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I.

• Specifications:

  Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

• UniProt:

  P35575

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 123-149 from the human protein was used as the immunogen for the G6PC antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, WB, IHC-P

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the G6PC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This G6PC antibody is available for research use only.

• Storage Conditions:

  Aliquot the G6PC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the G6PC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Western blot testing of human K562 cell lysate with G6PC antibody. Predicted molecular weight ~41 kDa.