RNF4 Antibody

• Description:

  The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.

• Specifications:

  Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

• UniProt:

  P78317

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 95-123 from the human protein was used as the immunogen for the RNF4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, FACS, IHC-P

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the RNF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This RNF4 antibody is available for research use only.

• Storage Conditions:

  Aliquot the RNF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the RNF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  IHC testing of FFPE human heart tissue with RNF4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.