TNFR1-associated DEATH domain protein Antibody / TRADD

• Description:

  The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq].

• Specifications:

  Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt:

  Q15628

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 126-151 from the human protein was used as the immunogen for the TRADD antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IF, FACS, IHC-P, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the TRADD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This TRADD antibody is available for research use only.

• Storage Conditions:

  Aliquot the TRADD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the TRADD antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  IHC testing of FFPE human breast carcinoma tissue with TRADD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.