COL8A2 Antibody

• Description:

  This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2.

• Specifications:

  Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000

• UniProt:

  P25067

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 571-599 from the human protein was used as the immunogen for the COL8A2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC-P, IF, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the COL8A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This COL8A2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the COL8A2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the COL8A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) mouse Neuro-2a and 4) mouse C2C12 cell lysate with COL8A2 antibody. Predicted molecular weight ~67 kDa.