TRIM65 Antibody

• Description :

  TRIM65 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger.

• Specifications :

  Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt :

  Q6PJ69

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 327-355 from the human protein was used as the immunogen for the TRIM65 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  FACS, IF, IHC-P, WB

• Purity :

  Antigen affinity purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the TRIM65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This TRIM65 antibody is available for research use only.

• Storage Conditions :

  Aliquot the TRIM65 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the TRIM65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, nuclear

• Image Legend :

  IHC testing of FFPE human prostate carcinoma tissue with TRIM65 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.