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DENND1B Antibody

CAT: 0800-F54541-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54541-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Clathrin-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 and bind to clathrin and clathrin adaptor protein-2. Thus, connecdenns link RAB35 activation with the clathrin machinery.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000
UniProt
Q6P3S1
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 39-68 from the human protein was used as the immunogen for the DENND1B antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IHC-P, WB
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the DENND1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This DENND1B antibody is available for research use only.
Storage Conditions
Aliquot the DENND1B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the DENND1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of human NCI-H460 cell lysate with DENND1B antibody. Predicted molecular weight: 34~87 kDa (multiple isoforms) .

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